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| PTP-MEG2, protein tyrosine phosphatase-MEG2 |
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| (2nd PTP cloned from MEG01 megakaryocytic cell line) | ||
| Synonyms: | none |
| Genomic Designation: | PTPN9 |
| Chromosomal Location: | 15q24.2 |
| ESTs | yes |
| Mouse | yes |
| Predicted MW or ORF: | |
| Exp. Determined Mr: | 68 kDa |
| Post-translational Modif.: | none reported. |
| 3D Protein Structures: | none |
| Tissue Expression: | widely expressed, highest in brain, pancreas, leukocytes, particularly neutrophils, macrophages, and mast cells. |
| Reported Substrates: | Y83 of N-ethylmaleimide-sensitive factor (NSF). |
| Function(s): | regulates secretory vesicle biogenesis and homeostasis. |
| Human Disease Assoc.: | one reported case of autism with heterozygous loss of PTPN9 locus. |
| Comm. Avail. Reagents: | none known to us. |
| Reagents Avail. from us: | full-length and partial cDNAs in pGEX, pEF5HA, PEF3HA, polyclonal Ab. |
| Domain Organization |
| Amino Acid Sequence of Human ORF |
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References (in alphabetical order) Gjörloff-Wingren A, Saxena M, Han S, Wang X, Alonso A, Renedo M, Oh P, Williams S, Schnitzer J, Mustelin T. (2000). Subcellular localization of intracellular protein tyrosine phosphatases in T cells. Eur. J. Immunol. 30, 2412-21. Gu M, Warshawsky I, Majerus PW. (1992). Cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast SEC14p. Proc. Natl. Acad. Sci. USA 89, 2980-2984. Huynh H, Bottini N, Williams S, Cherepanov V, Musumeci L, Saito K, Bruckner S, Vachon E, Wang X, Kruger J, Chow CW, Pellecchia M, Monosov E, Greer PA, Trimble W, Downey GP, Mustelin T. (2004). Control of vesicle fusion by a tyrosine phosphatase. Nat. Cell. Biol. 6, 831-839. Huynh H, Wang X, Li W, Bottini N, Williams S, Nika K, Ishihara H, Godzik A, Mustelin T. (2003). Homotypic secretory vesicle fusion induced by the protein tyrosine phosphatase MEG2 depends on polyphosphoinositides in T cells. J. Immunol. 171, 6661-6671. Heneberg P, Draber P. (2002). Nonreceptor protein tyrosine and lipid phosphatases in type I fc(epsilon) receptor-mediated activation of mast cells and basophils. Int. Arch. Allergy Immunol. 128, 253-263. Review. Kruger, J.M., Fukushima, T., Cherepanov, V., Borregaard, N., Loeve, C., Shek, C., Sharma, K., Tanswell, A.K., Chow, C.-W. & Downey, G.P. (2002). Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides. J. Biol. Chem. 277, 26202628. Krugmann,S., Anderson, K.E., Ridley, S.H., Risso, N, McGregor, A., Coadwell, J., Davidson, K., Eguinoa, A., Ellson,C.D., Lipp,P., Manifava, M., Ktistakis, N., Painter,G., Thuring, J.W., Cooper, M.A., Lim, Z.-Y., Holmes, A.B., Dove, S.K., Michell, R.H., Grewal, A., Nazarian, A., Erdjument-Bromage,H., Tempst, P., Stephens, L.R. & Hawkins, P.T. (2002). Identification of ARAP3, a novel PI3K effector regulating both Arf and Rho GTPases, by selective capture on phosphoinositide affinity matrices. Mol Cell 9, 95108. Qi Y, Zhao R, Cao H, Sui X, Krantz SB, Zhao ZJ. (2002). Purification and characterization of protein tyrosine phosphatase PTP-MEG2. J. Cell. Biochem. 86, 79-89. Seimiya H, Sawabe T, Toho M, Tsuruo T. (1995). Phorbol ester-resistant monoblastoid leukemia cells with a functional mitogen-activated protein kinase cascade but without responsive protein tyrosine phosphatases. Oncogene 11, 2047-2054. Wang X, Huynh H, Gjorloff-Wingren A, Monosov E, Stridsberg M, Fukuda M, Mustelin T. (2002). Enlargement of secretory vesicles by protein tyrosine phosphatase PTP-MEG2 in rat basophilic leukemia mast cells and Jurkat T cells. J. Immunol. 168, 4612-4619. Xu MJ, Sui X, Zhao R, Dai C, Krantz SB, Zhao ZJ. (2003). PTP-MEG2 is activated in polycythemia vera erythroid progenitor cells and is required for growth and expansion of erythroid cells. Blood 102, 4354-4360. Zhao R, Fu X, Li Q, Krantz SB, Zhao ZJ. (2003). Specific interaction of protein tyrosine phosphatase-MEG2 with phosphatidylserine. J. Biol. Chem. 278, 22609-22614. |
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